Open AccessComparative study of the genetic diversity of Mycobacterium tuberculosis Complex by Simplified Amplified Fragment Length Polymorphism and Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Analysis
Author(s) -
Patricia Jiménez,
María José Lahiguera,
Rafael Borrás,
Concepción Gimeno Cardona,
Marcelo Grijalva Silva,
María José Vallejo López,
María del Remedio Guna Serrano
Publication year - 2018
Publication title -
revista ecuatoriana de medicina y ciencias biológicas (remcb)/revista ecuatoriana de medicina y ciencias biológicas (remcb) (en línea)
Language(s) - English
Resource type - Journals
eISSN - 2477-9148
pISSN - 2477-9113
DOI - 10.26807/remcb.v39i1.568
Subject(s) - amplified fragment length polymorphism , genotyping , genotype , variable number tandem repeat , biology , mycobacterium tuberculosis , genetic diversity , genetics , tuberculosis , medicine , population , gene , pathology , environmental health
Species within the Mycobacterium tuberculosis (MTB) Complex are genetically monomorphic, hence the need of genotyping methods for a comprehensive understanding of the epidemiology of the disease. The genetic diversity of a Spain collection of eighty-three, GenoType MTBC® -confirmed Mycobacterium tuberculosis clinical isolates was assessed by simplified AFLP and 15-loci MIRU-VNTR. AFLP results showed 7 patterns (P1-P7), Dice´s coefficient was 71% for P1 vs P7 and 96% for P1 vs P2 and P2 vs P4. MIRU-VNTR showed 25 unique patterns and 14 clusters. Lineages found were as follows: Haarlem (23, 36.51%), Cammeroon (2, 3.17%), LAM (12, 19.05%), West African (6, 9.52%) and EAI (1, 1.59%). Discrimination indexes were 0.61 for AFLP and 0.92 for MIRU-VNTR. In conclusion, MIRU-VNTR is robust and reproducible for MTB genotyping. Simplified AFLP is a relatively easy-to-perform approach that might be useful for screening of isolates or in low resource settings.