Open AccessEvaluation of serodiagnostic tests for T.b. gambiense human African trypanosomiasis in southern Sudan
Author(s) -
Elrayah Ie,
Rhaman Ma,
Karamalla Lt,
Khalil Km,
P. Büscher
Publication year - 2007
Publication title -
eastern mediterranean health journal/eastern mediterranean health journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.442
H-Index - 47
eISSN - 1687-1634
pISSN - 1020-3397
DOI - 10.26719/2007.13.5.1098
Subject(s) - serology , trypanosomiasis , african trypanosomiasis , latex fixation test , virology , direct agglutination test , agglutination (biology) , antibody , filter paper , medicine , biology , immunology , chromatography , chemistry
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates from blood dried on filter paper, the LATEX test on diluted plasma and an ELISA on diluted plasma and filter paper. The specificities of all the serological tests were not significantly different from CATT on diluted blood (99.5%). The specificity of CATT on diluted blood was similar (99.3%). The highest sensitivities (100%) were observed with CATT on diluted blood and with CATT and LATEX on diluted plasma. CATT on diluted blood was more cost-efficient than the classic test, CATT on whole blood.