Open AccessMolecular evidence of mixed P. vivax and P. falciparum infections in northern Islamic Republic of Iran
Author(s) -
Sedigheh Zakeri,
Shadi Mamaghani,
Akram Abouie Mehrizi,
Zahra Shahsavari,
Rasheed Ahmad,
Shahnam Arshi,
Navid Dinparast Djadid
Publication year - 2004
Publication title -
eastern mediterranean health journal/eastern mediterranean health journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.442
H-Index - 47
eISSN - 1687-1634
pISSN - 1020-3397
DOI - 10.26719/2004.10.3.336
Subject(s) - plasmodium vivax , plasmodium falciparum , malaria , nested polymerase chain reaction , polymerase chain reaction , vivax malaria , islamic republic , virology , biology , immunology , gene , islam , genetics , geography , archaeology
This study compared basic microscopy with molecular detection of Plasmodium species. According to thick-film microscopy, 100% of 142 malaria cases in Pars-Abad, Ardebil province, were infected with a single species, P vivax. However, nested polymerase chain reaction [PCR] detected mixed species infections of both P. vivax and P. falciparum in 7.0%. In Maz and eran province, 2/20 blood films were diagnosed with only P. falciparum and 18/20 with only P. vivax. However, nested PCR detected 17/20, 2/20 and 1/20 with P. vivax only, P. falciparum only and mixed species respectively. The unexpected presence of P. falciparum urges prompt investigation and immediate treatment of malaria cases in this region