Open AccessA panel of eight tests in the serodiagnosis and immunological evaluation of acute brucellosis
Author(s) -
Wafer Dabdoob,
Zainalabideen A. Abdulla
Publication year - 2000
Publication title -
eastern mediterranean health journal/eastern mediterranean health journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.442
H-Index - 47
eISSN - 1687-1634
pISSN - 1020-3397
DOI - 10.26719/2000.6.2-3.304
Subject(s) - brucellosis , direct agglutination test , brucella melitensis , medicine , brucella , agglutination (biology) , immunology , test (biology) , rose bengal , brucella abortus , antibody , virology , serology , biology , paleontology , genetics
A panel of eight tests was used to study 200 cases of acute brucellosis, 200 patients negative for brucella as a control group and 200 apparently healthy individuals as a second control group. The best diagnostic test was the rose Bengal test using an imported reagent [BioMerieux, France] and 2 local reagents. This test was improved from being a screening test to be a titrable one. The best two tests used together were the tube agglutination test with Coomb-like test. The indirect fluorescent antibody test had no advantages over the use of other tests. The 2-mercaptoethanol test and C-reactive protein test were useful in checking the brucellosis activity. Normal numbers of E-rosette forming cells and inefficient neutrophils in phagocytosis were found in peripheral blood during acute brucellosis