The The Effect of Elution Volume for Immunoprecipitation on m6A-Seq Analysis | Zendy

Yuhui Xu | Zendy; Lize Shen | Zendy; Guolin Li | Zendy

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The The Effect of Elution Volume for Immunoprecipitation on m6A-Seq Analysis

Author(s) -

Yuhui Xu,

Lize Shen,

Guolin Li

Publication year - 2021

Publication title -

proceedings of anticancer research

Language(s) - English

Resource type - Journals

eISSN - 2208-3553

pISSN - 2208-3545

DOI - 10.26689/par.v5i4.2239

Subject(s) - elution , immunoprecipitation , chromatography , volume (thermodynamics) , chemistry , consumption (sociology) , significant difference , mathematics , statistics , physics , biochemistry , gene , social science , quantum mechanics , sociology

Objective: To develop a cost-effective method to reduce the time consumption of elution in immunoprecipitation. Method: Two volumes (125 ?L for Group C and 100 ?L for Group T) of elution buffer were used to explore whether smaller volume could save testing time. Result: Time consumption of elution in Group T was significantly shorter than that in Group C, while the efficiency of eluted m6A-containing fragments and the performance of m6A-Seq as indicated by m6A peak distributions showed no difference between the two groups. Conclusion: A smaller volume of elution buffer was an economical way to reduce time consumption in immunoprecipitation.

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