Open AccessThe The Effect of Elution Volume for Immunoprecipitation on m6A-Seq Analysis
Author(s) -
Yuhui Xu,
Lize Shen,
Guolin Li
Publication year - 2021
Publication title -
proceedings of anticancer research
Language(s) - English
Resource type - Journals
eISSN - 2208-3553
pISSN - 2208-3545
DOI - 10.26689/par.v5i4.2239
Subject(s) - elution , immunoprecipitation , chromatography , volume (thermodynamics) , chemistry , consumption (sociology) , significant difference , mathematics , statistics , physics , biochemistry , gene , social science , quantum mechanics , sociology
Objective: To develop a cost-effective method to reduce the time consumption of elution in immunoprecipitation. Method: Two volumes (125 ?L for Group C and 100 ?L for Group T) of elution buffer were used to explore whether smaller volume could save testing time. Result: Time consumption of elution in Group T was significantly shorter than that in Group C, while the efficiency of eluted m6A-containing fragments and the performance of m6A-Seq as indicated by m6A peak distributions showed no difference between the two groups. Conclusion: A smaller volume of elution buffer was an economical way to reduce time consumption in immunoprecipitation.