Open AccessResearch and Development of Butterfly Microfluidic Gene Chip for 19 Common Pathogenic Microorganisms of Nosocomial Infection
Author(s) -
Jingjing Yu,
Zhifeng Xu,
Jinhai Tian,
Rong Ma,
Qi Huang,
Jia Cao,
Jia Wang,
Libin Wang
Publication year - 2022
Publication title -
journal of clinical and nursing research
Language(s) - English
Resource type - Journals
eISSN - 2208-3693
pISSN - 2208-3685
DOI - 10.26689/jcnr.v6i1.2912
Subject(s) - microfluidic chip , microfluidics , pathogenic bacteria , microorganism , chip , gene , microbiology and biotechnology , biology , nanotechnology , materials science , bacteria , computer science , genetics , telecommunications
Based on butterfly microfluidic gene chip technology, a method for rapid, accurate and efficient detection of 19 common pathogenic microorganisms of nosocomial infection was established, and a butterfly microfluidic gene chip with high-throughput detection was designed and fabricated. Using constant temperature amplification technology, using the polymerase with chain replacement function to react at constant temperature (65?) and combined with microfluidic chip technology, primers were designed according to the target genes of 19 pathogenic microorganisms, and a butterfly microfluidic gene chip which can detect 19 common pathogenic microorganisms of nosocomial infection was made to simplify the inspection operation process and verify the sensitivity of the chip. The butterfly microfluidic gene chip can be used for the rapid and efficient detection of 19 common pathogenic microorganisms of nosocomial infection, and provides a new idea for the detection and auxiliary diagnosis of pathogenic microorganisms of nosocomial infection.