Open AccessEffect of Rat C6 Glioma Cells in Varying Concentrations of Cultured in Isorhamnetin
Author(s) -
Rui-rui Tian
Publication year - 1970
Publication title -
journal of clinical and nursing research
Language(s) - English
Resource type - Journals
eISSN - 2208-3693
pISSN - 2208-3685
DOI - 10.26689/jcnr.v1i1.98
Subject(s) - isorhamnetin , glioma , apoptosis , flow cytometry , in vitro , cell , blot , cell growth , chemistry , cell culture , microbiology and biotechnology , biology , biochemistry , cancer research , flavonoid , antioxidant , kaempferol , genetics , gene
Objective: To investigate the effects of different concentrations of isorhamnetin on C6 rat glioma cells in vitro from January 2015 to June 2015. Methods: The blank control group, blank solvent control group and four concentration groups were used to observe the cell growth status under a microscope. MTT colorimetric assay was used to detect the effect of isorhamnetin on C6 glioma cells in vitro and the cell inhibition rate And survival rate were measured. The apoptotic and apoptotic rates were measured by flow cytometry in the treatment group and the control group. The relationship between the different concentrations of isorhamnetin and C6 glioma cell apoptosis was analyzed the total protein was extracted and the total AKT protein and Ser473 AKT protein content were detected by Western blotting. The rat model of glioma was constructed by SD rats.Five days of isorhamnetin was continuously fed and the plasma was detected by high-performance liquid chromatography,liver, brain tissue isorhamnetin content.Â