Open AccessEffect of Recombinant Human Lactoferrin on Allergic Rhinitis in Mice Nasal Drip and Its Mechanism
Author(s) -
WANG Xiao-li
Publication year - 1970
Publication title -
journal of clinical and nursing research
Language(s) - English
Resource type - Journals
eISSN - 2208-3693
pISSN - 2208-3685
DOI - 10.26689/jcnr.v1i1.95
Subject(s) - lactoferrin , ovalbumin , mucous membrane of nose , nasal cavity , immunology , recombinant dna , allergy , infiltration (hvac) , medicine , chemistry , immune system , gene , biochemistry , physics , surgery , thermodynamics
Objective: To study the effect of recombinant human lactoferrin on allergic rhinitis in mice nasal drip and explore its possible molecular mechanism. Methods: The animal model of allergic rhinitis in BALB / c mice was established by ovalbumin. The eosinophils, goblet cells and mast cell infiltration of nasal mucosa were analyzed by hematoxylin-eosin,happening. The transcription factors and cytokines and expression levels of lactoferrin gene and protein of T lymphocyte subsets in nasal cavity were detected by fluorescence quantitative RT-PCR and enzyme-linked immunosorbent assay. Spearman was used to analyzing the relationship between lactoferrin expression levels and eosinophils in the nasal cavity.Â