Open AccessDetection And Molecular Characterization of Theileria Ovis in Sheep And Goats with Clinical The Ileriosis in Kurdistan, Iraq
Author(s) -
Zuber Ismael Hassen,
Azad A. Meerkhan
Publication year - 2020
Publication title -
maǧallaẗ ǧāmi'aẗ duhūk
Language(s) - English
Resource type - Journals
eISSN - 2521-4861
pISSN - 1812-7568
DOI - 10.26682/sjuod.2020.23.2.8
Subject(s) - theileria , ovis , veterinary medicine , biology , livestock , virology , medicine , parasite hosting , ecology , world wide web , computer science
This study was carried out to detect Theileria infection in sheep and goats in Kurdistan region, Iraq from June 2019 to April 2020. Molecular method was used to identify Theileria species. Sixty- seven blood samples were taken from 45 sheep and 22 goats based on clinical signs of theileriosis during tick activating season. The 67 samples were PCR edm and as a result, 20 species-specific PCR were positives (26.67% (12/20) were Theileria ovis in sheep and 36.36% (8/20) were from goats). The results of the gene analysis in the current study were registered in NCBI under four accession numbers (MN889986, MN889987, MN889988 and MN889989), which shows that sheep and goats can be infected with Theileria ovis. This is the first report of Theileria species in goats with clinical theileriosis in Kurdistan, so the gene flow and disease transmission between sheep and goats is most expected. PCR is a useful diagnostic tool to detect ovine theileriosis with a single test and suggested that T. ovis is the dominant piroplasmid agent in Erbil. In addition, it revealed that sheep is very susceptible to theileriosis than goats