Open AccessThe Response of in Vitro Propagation of Marumi Kumquat (fortunella Japonica Thunb.) to Different Culture Media, Plant Growth Regulators And Different Fructose Concentration
Author(s) -
Jeillan Hussein,
Diaa ibraheam
Publication year - 2020
Publication title -
maǧallaẗ ǧāmi'aẗ duhūk
Language(s) - English
Resource type - Journals
eISSN - 2521-4861
pISSN - 1812-7568
DOI - 10.26682/csjuod.2020.23.1.19
Subject(s) - explant culture , shoot , sterilization (economics) , fructose , horticulture , japonica , biology , in vitro , micropropagation , murashige and skoog medium , botany , food science , biochemistry , economics , monetary economics , foreign exchange market , foreign exchange
Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%