Open AccessBanana resistant starch inhibitory inflammation and cyclooxygenase-2 in BALB/c mice induced by azoxymethane and dextran sodium sulfate
Author(s) -
Syafira Noor Pratiwi,
Diaur Afifah,
Nyoman Suci Widyastiti,
Vega Karlowee,
Gemala Anjani,
Hermawan Istiadi
Publication year - 2022
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.6(2).262
Subject(s) - azoxymethane , inflammation , cyclooxygenase , staining , immunohistochemistry , starch , h&e stain , resistant starch , medicine , chemistry , enzyme , pathology , biochemistry , colorectal cancer , cancer
Inflammation in colonic tissue is one of the earliest developmental pathways in colorectalcancer and proteins such as cyclooxygenase are crucial to this inflammation. Batu bananasand kepok bananas contain resistant starch which can inhibit inflammation and COX-2 inmice colon that are induced by Azoxymethane and Dextran Sodium Sulfate. This studywas to observe the effect of banana flour on inflammation and cyclooxygenase enzyme incolonic tissue. This study involved 20 BALB/c mice in 4 groups, negative control group,positive control group, batu banana treatment group, and kepok banana treatment group.The level of inflammation was seen from the colon tissue treated with hematoxylin eosinwhile Cyclooxigenase-2 (COX-2) with COX-2 immunohistochemical staining. Thespecimens were viewed under a light microscope at a magnification of 400 times. Thetreatment groups with resistant starch of batu and kepok banana flour had a significantlylower level of inflammation when compared to the positive group (p = 0.035). The COX-2score in the treatment group resistant starch of batu and kepok banana was significantlylower than the positive control group (p<0.001). The COX-2 intensity in both groups waslower than the positive group but not significant (p<0.001). The combined score betweenthe percentage and the intensity of COX-2 expression in the two treatment groups wasalso had lower than the positive control group (p<0.001). Resistant starch of batu andkepok banana can inhibit inflammation and suppressed the expression of COX-2.