Open AccessProtective effect of two Thai pigmented rice cultivars against H2O2-induced oxidative damage in HT-29 cell culture
Author(s) -
P. Boonyanuphong,
U. Tobgay
Publication year - 2022
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.6(1).206
Subject(s) - antioxidant , reactive oxygen species , oxidative stress , red rice , black rice , chemistry , dna damage , viability assay , food science , cultivar , oxidative damage , biochemistry , biology , botany , cell , dna , raw material , organic chemistry
Radicals derived from exogenous and endogenous sources are considered to be theprincipal cause of genetic damage. Exogenous and endogenous radicals participate in thereactive oxygen species (ROS) formation, which leads to damages in the DNA, RNA,proteins and lipids. However, dietary compounds, mainly from pigmented rice, are anessential source of antioxidants that help protect cells from damage. This study seeks todetermine the antioxidant properties and cytoprotective effect of two Thai pigmented riceextracts namely the glutinous black rice (native name: Neaw dum moa37) and red rice(native name: Hom gradung-nga57) on H2O2-induced damage in HT-29 cells. Thebioactive compound contents, as well as antioxidant activities of both rice extracts, wereinvestigated. The protective effect of rice extracts on H2O2-induced damage was executedfollowing the co-incubation method. HT-29 cells were exposed to H2O2 and different riceextract concentrations for 3 h and an MTT assay was used to measure the viability of thecell. The ROS level was determined using the 2′,7′-dichlorofluorescin diacetate (DCFDA). The result showed that glutinous black rice extract contained significantly highercontents of all analysed antioxidants and activities than red rice extract. Glutinous blackrice showed a higher cytotoxic effect compared to red rice. At the non-toxic concentrationof both rice extracts, the HT-29 cells were guarded against the H2O2 induced oxidativestress. Besides, the intracellular ROS accumulation result from H2O2 exposure wassignificantly reduced in the presence of rice extracts for both glutinous black rice and redrice compared to control. Hence, this study has demonstrated the potential properties ofboth pigmented rice extracts in alleviating H2O2-mediated damage in HT-29 cells.