Open AccessOptimization of selected plant growth regulators on callus induction of Oryza sativa L. var MR 219
Author(s) -
H Siti Nur Adila,
Nor Azma Yusuf
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(s4).011
Subject(s) - callus , kinetin , browning , oryza sativa , sucrose , biology , botany , horticulture , murashige and skoog medium , tissue culture , chemistry , in vitro , food science , biochemistry , gene
MR 219, an indica rice from a cross between MR 137 and MR 151 is a long-grain ricevariety of high-yielding, good quality of shape and taste, short maturation as well asresistance to blast and bacterial leaf blight. However, it is regarded as a recalcitrant varietydue to its low ratio of callogenesis and regeneration in in vitro culturing. This would be ahindrance to crop improvements such as genetic transformation and other cropimprovement methods. The objective of this study was to optimize the concentrations andcombinations of plant growth regulators (PGRs) which were 2,4-Dichlorophenoxyaceticacid (2,4-D), Kinetin (KIN) and Naphthalene acetic acid (NAA) on its callus inductionthat ranging from 0.5 to 15.0 mg/L. The callus was induced from the mature seeds of MR219 on the Murashige and Skoog (MS) media supplemented with 4.4 g/L of MS powderwith vitamins, 30.0 g/L of sucrose and 3.5 g/L of gelrite with the addition of 2,4-D, KINand NAA in different concentrations and combinations. All media supplemented with 2,4-D had successfully induced the callus and 2.0 mg/L of 2,4-D was the best concentrationfor the callus induction with a 100% success rate. The addition of 15.0 mg/L of NAA,provided less time taken for callus induction with better callus morphology which resultedin fewer browning problems.