Open AccessEvaluation of total phenolic content, antioxidant activity, germination power, and yield of pigeon pea (Cajanus cajan) sprouts elicited using various Naalginate levels with different elicitation duration
Author(s) -
Setyaningrum Ariviani,
K.C. Hapsari,
Gusti Fauza,
Dwi Ishartani,
Windi Atmaka,
Lia Umi Khasanah,
Siswanti
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(s2).005
Subject(s) - cajanus , germination , antioxidant , food science , chemistry , trolox , trolox equivalent antioxidant capacity , antioxidant capacity , horticulture , biology , biochemistry
This study was aimed to investigate the total phenolic content and antioxidant activity ofelicited pigeon pea (Cajanus cajan) sprouts prepared using various Na-alginateconcentrations at different elicitation durations. The germination power and yield of thesprouts were also examined. Elicitation proved capable to improve the antioxidantcapacity of legumes sprouts but the yield and germination rates were decreased due to theelicitation stress. In this study, elicitation conducted by immersing pigeon pea seeds in Na-alginate solutions (250, 300, and 350 ppm) for various duration (16, 20, and 24 hrs). Theseeds were then germinated for 48 hrs to produce Na-alginate elicited pigeon pea sprouts.The results showed that the total phenolic content (TPC) and antioxidant activity (Troloxequivalent antioxidant capacity, TEAC, and Ferric reducing antioxidant power, FRAP) ofelicited pigeon pea sprouts were significantly increased along with the increasingelicitation duration in all Na-alginate levels. The higher Na-alginate levels produce ahigher level of TPC, TEAC, and FRAP values. On the other hand, germination power andthe yield of the sprouts were significantly decreased along with increasing Na-alginatelevels and elicitation duration. Elicitation using 350 ppm Na-alginate with an elicitationduration of 24 hrs produces elicited pigeon pea sprouts with the highest TPC andantioxidant activity, but lowest germination power and yield. These results have animportant consequence in developing an elicitation technique to improve the antioxidantcapacity of leguminous.