Open AccessAcute oral toxicity of squid and cuttlefish ink powder enzyme hydrolysates in Sprague-Dawley rats in accordance with the OECD Test Guideline 425
Author(s) -
Z Ayu Shazwani,
T Husnul Azan,
M. F. Wan Ezumi,
Rabeta
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(5).219
Subject(s) - hydrolysate , toxicity , cuttlefish , acute toxicity , squid , medicine , pharmacology , body weight , food science , toxicology , physiology , chemistry , biology , fishery , biochemistry , hydrolysis
The objectives of this study were to determine LD50 and establish the safety of ink squidand cuttlefish hydrolysates. In the acute toxicity study, three groups of female rats wererandomly assigned. One group served as the control and two groups orally received asingle limiting dose (2000 mg/kg body weight) of ink hydrolysates. There were no signsof adverse toxicity observed in behavioural patterns, clinical signs, and no significantdifferences (p>0.05) between the control and treated rats regarding their food and waterconsumption and body weight for up to 14 days. The histopathological evaluationrevealed a normal structure and the absence of noticeable lesions in the vital organs oftreated animals. It can be concluded that LD50 value is greater than 2000 mg/kg. Theresults showed that the squid ink powder enzyme hydrolysate (SIPEHs) and cuttlefish inkpowder enzyme hydrolysate (CIPEHs) possess low toxicity, as indicated in the rat model.The preliminary results suggested that it should be further evaluated for long-term use andrepeated dose effects to support the safe use of these hydrolysates.