Open AccessEffect of different extraction solvents on antioxidant properties of water lily (Nymphaeaceae antares) flower petal and stamen
Author(s) -
Z. Mohd Zin,
A.Z.M. Sanuri,
Nur Alwani Ali Bashah,
K. M. S. Ibrahim,
Hafiz Yahya,
M.K. Zainol
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(3).653
Subject(s) - ethyl acetate , chemistry , dpph , petal , gallic acid , chromatography , rutin , quercetin , kaempferol , flavonoid , botany , organic chemistry , antioxidant , biology
Water lily (Nymphaeaceae antares) contains a vast array of phenolic compounds withimportant natural antioxidative properties that could inhibit the adverse effects of thereactive oxygen species produced in living things. These substances are complex incomposition which requires a suitable extraction medium to exploit the phenoliccompounds. This study was designed to determine the antioxidative properties in petal andstamen of water lily extracted using different extraction solvents namely ethyl acetate and60% methanol. Antioxidant properties of the samples were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric thiocyanate (FTC) method,thiobarbituric acid (TBA) test, total phenolic content (TPC), total flavonoid content (TFC)and total tannin content (TTC) along with individual flavonoids content using HighPerformance Liquid Chromatography (HPLC). The yield of extract was found to be thehighest in petals extracted with 60% methanol (44.55±1.63%) while stamen extracted withethyl acetate yielded the lowest (24.50±1.09%). In the DPPH inhibition method, petalextracted with ethyl acetate obtained the highest scavenging activity (89.15±3.62%). FTCanalysis revealed that ethyl acetate extract of petal experienced the highest percentageinhibition (76.70±6.48%) while in TBA analysis ethyl acetate extract of stamen exhibitedthe highest percentage inhibition (70.78±1.39%). Flavonoids such as quercetin,kaempferol, rutin, gallic acid, catechin, epigallocatechin, p-coumaric acid and myricetinwere found to be present in ethyl acetate and 60% methanol extracts of water lilies stamenand petal. The information from this study indicates that water lily petals and stamens maybe integrated as a good source of nutrients in the food industry, the pharmaceuticalindustry. If the advantages of water lily are not used and integrated into everyday life forhealth benefits, it will be a massive waste of natural resources.