Open AccessIdentification of bacteriological quality and antimicrobial resistance of microorganisms isolated from animal foods collected from the abattoir, butcher shops and local seafood market
Author(s) -
P. Vijayalakshmi,
A. Rajani Chowdary,
Vidyullatha Pellakuri,
M. Sharon Sushma
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(3).570
Subject(s) - butcher , biology , salmonella , antibiotic resistance , antimicrobial , veterinary medicine , raw meat , multiple drug resistance , microbiology and biotechnology , food science , bacteria , drug resistance , antibiotics , medicine , geography , genetics , archaeology
The current study aimed to isolate bacteria that harbour various animal food products likemeat, chicken and seafoods collected from the abattoir, butcher shops and local seafoodmarket and to determine the antimicrobial resistance pattern of isolated pathogens whichare responsible for various foodborne illnesses in human beings. A total of forty rawanimal product samples were collected from the abattoir, butcher shops and local seafoodmarket of Visakhapatnam. The samples selected for the study include raw chicken, meat,crab, prawns and different varieties of fish. A classic random sampling technique wasemployed to collect the study samples. All the samples were processed immediately usingstandard microbiological protocols. The bacteria isolation and characterization were doneby studying morphological characteristics with staining methods, cultural characteristicsby isolating and growing the pathogenic microorganisms in various selective anddifferential culture media. Antimicrobial susceptibility testing was performed by the Kirby-Bauer method by following Clinical and Laboratory Standards Institute (CLSI)guidelines. EDTA-Disc Potentiation Test and Imipenem-EDTA Double disc synergy testare used to detect the metallo beta-lactamase production of isolated pathogens. Thehighest number of isolates belong to Salmonella species (18), Pseudomonas aeruginosa(18) followed by Vibrio species (14) and few isolates belong to Enterobacter species (4).Majority of the microbial isolates obtained in the current study were multidrug resistant.The isolates from the abattoir environments, slaughterhouses, fish markets were found toexhibit variable resistance pattern to aminoglycosides, macrolides, β-lactams,cephalosporins, quinolone antibiotics used in the present study and at the same time mostof them were sensitive to carbapenem antibiotic imipenem. Antimicrobial resistance(AMR) prevents the designing and assessment of effective interventions. If such a link canbe established, then the tracking of antibiotic use and consumption data could befurthermore used as a surrogate indicator for the risk of potential antibiotic resistance(ABR) emergence.