Open AccessProtective effect of moringa-based beverages against hyperlipidemia and hyperglycemia in type 2 diabetes-induced rats
Author(s) -
Muath M. Al Zhrani,
Sami A. Althwab,
Thamer Aljutaily,
Hani A. Alfheeaid,
I. S. Ashoush,
Hassan Barakat
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(2).536
Subject(s) - moringa , chemistry , antioxidant , malondialdehyde , food science , ascorbic acid , alloxan , caffeic acid , diabetes mellitus , hyperlipidemia , traditional medicine , lipid profile , biochemistry , medicine , endocrinology , cholesterol
The objectives of this study were to determine the phenolic profile and potentialantioxidant activity of Moringa oleifera leaves (MOL) cultivated in Saudi Arabia and toinvestigate the protective effect of moringa-based beverages on lipidemic and glycemicparameters in alloxan-induced diabetic rats (DR). The total phenolic content (TPC),chlorophylls, total carotenoids, total flavonoids, total flavonols, ascorbic acid content, andantioxidant activities (AOA) of MOL were determined and HPLC analysis of MOL wasquantitatively performed. Moringa beverages as pure moringa (PM) and ginger-flavoredmoringa (GM) were given to diabetic rats (DR) at a dose of 1 mL 100 g-1body weight/dayfor a successive six weeks. Glucose level (GL), lipid profile, and antioxidant parametersincluding malondialdehyde (MDA), reduced glutathione (GSH), and pancreashistopathological alterations were examined. Results indicated that MOL and preparedmoringa-based beverages possess a suppressive amount of phenolics with considerableantioxidant activity in vitro. TPC was 78.72 mg GAE g-1 DW possess AOA as 36.33 μmolTE g-1 DW. Among the eleven identified phenolic acids and six flavonoids in MOL,caffeic acid and catechin were predominant and recorded 151.01 and 736.30 mg/100 g,respectively. Administration of PM or GM beverages significantly decreased GL andsignificantly improved serum lipid profiles in the DR group. Interestingly, oraladministration of PM and GM beverages significantly attenuated the MDA and GSHlevels in the DR compared to normal rats. Histopathologically, severe atrophy was notedin the islets of Langerhans in the DR group. However, after six weeks of oraladministration of PM beverage, only mild atrophy was recorded in the islets ofLangerhans, while with GM beverage, no histopathological alteration in the islets ofLangerhans was found. The nutritional and biological evaluation of the moringa-basedbeverages revealed that both PM and GM beverages exhibit high antioxidant activity,possess antihyperglycemic and antihyperlipidemic effects, and alleviated alloxan-inducedpancreatic damage in DR. Moringa could be recommended for diabetes and utilized for itsgreat therapeutic benefits.