Open AccessAntioxidant activities and simultaneous HPLC-DAD profiling of polyphenolic compounds from Moringa oleifera Lam. Leaves grown in Bangladesh
Author(s) -
K.S. Ahmed,
Israt Jahan,
Ferdoushi Jahan,
H. Hosain
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(1).410
Subject(s) - moringa , chemistry , polyphenol , dpph , rosmarinic acid , cinnamic acid , syringic acid , quercetin , ferulic acid , rutin , antioxidant , catechin , food science , gallic acid , traditional medicine , biochemistry , medicine
Moringa oleifera Lam. has been used as a traditional medicine for the treatment of severaldiseases. In this study, the antioxidant activity and simultaneous HPLC profiling ofpolyphenolic compounds in 80% ethanol and water extracts of M. oleifera tender andmature leaves available in Bangladesh were investigated. The 80% ethanol extract of M.oleifera tender leaves contained high concentration and more amount of 3,4-dihydroxybenzoic acid, (-) epicatechin and rosmarinic acid (201.32±1.94, 213.08±1.96and 133.84±1.42 mg/100 g of dry extract, respectively) than that of mature leaves(82.55±1.09, 141.86±1.10 and 16.23±0.76 mg/100 g of dry extract, respectively). On theother hand, the water extract of M. oleifera tender leaves contained a high concentration ofcatechin hydrate, trans-ferulic acid and quercetin (530.05±1.91, 166.38±0.90 and771.74±1.34 mg/100 g of dry extract, respectively) than that of mature leaves(207.21±0.90, 62.42±0.45 and 483.25±1.04 mg/100 g of dry extract, respectively). It wasalso found that 3,4-dihydroxybenzoic acid, catechol, p-coumaric acid and trans-cinnamicacid were detected only in 80% ethanol extract of M. oleifera leaves but syringic acid wasidentified in the water extract. In DPPH free radical scavenging activity test, IC50 valuesof 80% ethanol extract of tender leaves (EETL), 80% ethanol extract of mature leaves(EEML), water extract of tender leaves (WETL) and water extract of mature leaves(WEML) were found to be moderately significant (105.50±1.05, 115.00±1.14,216.00±1.34 and 269.50±1.56 μg/mL, respectively) when compared to the IC50 value ofascorbic acid, 3.32±0.07 μg/mL. The polyphenolic compounds are responsible forantioxidant activity. On the basis of the present study, it can be suggested that the leavesshow antioxidant activity and it’s contained a significant amount and different types ofpolyphenolic compounds. Standardized polyphenolic compounds from M. oleifera leavescould be used as a better source of natural antioxidant supplement.