Open AccessHigh-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea
Author(s) -
K.S. Ng,
Z. Mohd Zin,
N. MohdMaidin,
Hasmadi Mamat,
Nurul Hanisah Juhari,
M.K. Zainol
Publication year - 2020
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(1).311
Subject(s) - high performance liquid chromatography , chemistry , rutin , kaempferol , gallic acid , chromatography , decoction , catechin , quercetin , herbal tea , traditional medicine , food science , antioxidant , polyphenol , organic chemistry , medicine
Natural plant products are becoming more and more essential in helping to promote safewell-being worldwide. This leads to a substantial rise in the consumption of various herbalteas. The presence of beneficial bioactive ingredients such as flavonoids may be correlatedwith Napier grass herbal tea having health benefits associated with their intake. Studieshave shown that herbal teas have preferentially high antioxidant activity due to thepresence of flavonoids in them. The purpose of this study was to identify the Napier grassherbal teas flavonoids prepared under different conditions. Napier grass herbal teas havebeen formulated using green tea and black tea processes, respectively. The tea sampleswere extracted in water (95°C, 30 mins) and 60% (v/v) aqueous methanol (30 mins),respectively. Approximately, 1% (v/v) aqueous acetic acid solution (solvent A) andacetonitrile (solvent B) were used as the mobile phase. The flow rate was adjusted to 0.7mL/min, the column was thermostatically controlled at 28°C, and the injection columnwas kept at 20 μL. HPLC chromatograms were detected using a photodiode array UVdetector at 272 nm. Gallic acid, P-coumaric acid, catechin, epigallocatechin gallate,quercetin, rutin, myricetin and kaempferol were found in both Napier grass water andmethanolic extracts, respectively. The findings suggested that the HPLC techniques areideal for the detection and identification of flavonoids in Napier grass teas