Open AccessEffects of extraction conditions on characterization of gelatin from water buffalo (Bubalus bubalis) skin
Author(s) -
R Rabiatul Amirah,
A J Ellya Hazreera,
M N Nor Qhairul Izzreen,
Ashari Rozzamri,
M R Umi Hartina
Publication year - 2020
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.4(s6).015
Subject(s) - gelatin , bubalus , extraction (chemistry) , chemistry , absorption (acoustics) , chromatography , absorption of water , nuclear chemistry , materials science , biochemistry , biology , ecology , composite material
The study aimed to determine the characteristics of gelatin from water buffalo (Bubalusbubalis) skin pre-treated with NaOH and Ca(OH)2 at different concentrations (0.3 M, 0.5M and 0.7 M) and extracted at 65˚C for 6 hrs and 24 hrs respectively. The gelatin obtainedwas evaluated for its moisture, protein and ash content, UV-vis absorption value, colour,emulsifying and foaming properties. The highest yield (20.25%) was observed for gelatinextracted by 0.5 M NaOH at 24 hrs extraction time. For alkaline pre-treatment, it wasfound that NaOH was more efficient than Ca(OH)2 in terms of preparing the skin forsubsequent extraction process. The protein content of the extracted gelatin samples was inthe range of 71.76% - 87.83%, showing that the varying processing conditions aresufficiently to recover protein from the raw material. Ash content for all samples was inagreement with USDA standard, which was below than 3%. The extracted gelatin hadvarying pH values which were from 5.47 to 7.02. The gelatin was colourless with ‘L’values of more than 80, except for 0.7 M Ca(OH)2 at 24 hrs which showed slightly darkerproperties. The intensity of the UV-vis absorption spectrum showed that a high absorptionpeak was observed at 6 hrs of extraction time (230 – 250 nm) compared to 24 hrsextraction time. Emulsifying properties of buffalo gelatin increased with increasingconcentrations of alkaline except for 0.7 M NaOH and 0.7 M Ca(OH)2 for both extractiontime. Meanwhile, foam expansion of the gelatin extracted from the different extractionconditions was observed to have a significant difference (p < 0.05) for all samples. To ourknowledge, buffalo skin has the potential to be an alternative source of gelatin in thediversified industrial application by modifying the extraction conditions in order toproduce gelatin with desired quality.