Open AccessEffect of extraction solvents on antioxidant and wound healing properties of Carica papaya leaves extract
Author(s) -
Husnul Hanani Soib,
Hend Ismail,
Harisun Yaakob,
Mohamad Khairul Hafiz Idris,
Aznida Firzah Abdul Aziz
Publication year - 2020
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.4(s2).s03
Subject(s) - carica , chemistry , antioxidant , dpph , extraction (chemistry) , polyphenol , solvent , ethanol , wound healing , caffeic acid , traditional medicine , quercetin , methanol , food science , chromatography , biochemistry , botany , organic chemistry , biology , medicine , immunology
Carica papaya is a well-known plant that has been reported to exert variouspharmacological activities including antioxidant and wound healing properties. However,to date, the lack of scientific evidence has been explored on the efficiency of the solventstowards C. papaya extract as a potential wound healer. The selection of proper extractionsolvent plays a pivotal role in extracting the bioactive compounds from the plant.Therefore, the present study was aimed to examine the effect of three types of extractionsolvents (methanol, ethanol and aqueous) on the antioxidant activity and wound healingpotential of C. papaya leaves. In this study, the effect of different solvents of C. papayaleaves extracts were determined through 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radicalscavenging activity, cytotoxicity assay and scratch migration assay on Human SkinFibroblast cells (HSF1184). The result showed that the methanolic extract of C. papayapossessed a significant antioxidant activity as compared to ethanol and aqueous extract.The result also demonstrated that methanolic extract significantly stimulates the highestmigration rate of HSF1184 cell at a concentration of 3.9 µg/mL, 7.8 µg/mL and 15.6 µg/mL (p 0.05) after 48 hrs with no cytotoxicity observed at the concentration lower than1000 mg/mL as compared to other solvents. HPLC analysis of methanol extract identifiedthe presence of two flavonoids (catechin, quercetin) and two phenolic acids (caffeic acid,cinnamic acid). The findings suggest that the methanolic extract of C. papaya is effectivein fighting free radicals and it has good wound healing activities. It also has the potentialto be further explored for its medicinal values owing to the abundance of bioactivecompounds from the extract.