Open AccessAntifungal activity of Piper cubeba L. extract on spoilage fungi isolated from tomato, grapes and lemon
Author(s) -
Z Fazly-Ann,
M Y Nor-Hafizah,
Yaya Rukayadi
Publication year - 2019
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.4(1).278
Subject(s) - penicillium citrinum , food spoilage , piper , fungicide , phytochemical , food science , coleus , minimum inhibitory concentration , piperaceae , traditional medicine , biology , antifungal , antimicrobial , chemistry , botany , microbiology and biotechnology , bacteria , medicine , genetics
The berries of Piper cubeba L. or commonly recognized as tailed pepper are traditionallyused as flavoring ingredients in various types of food. It has been reported with abundantof phytochemical compounds that contribute to its nutritional and medicinal properties.The antimicrobial effect of the spice including antifungal activity was believed to reducethe potential of fungal spoilage thus minimizing food losses and wastage. This study wasaimed to determine the antifungal activity of P. cubeba L. extract against isolated spoilagefungi namely Geotricum candidum (TMa 001), Penicillium citrinum (GRd 001) andTrametes hirsuta (LMd 001) by in vitro and in vivo techniques. Disc diffusion assay,minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC)test of the methanolic extract were performed on the selected fungi. The largest inhibitionzone, fungistatic and fungicidal effects were detected in T. hirsuta with 18.30±3.00 mm,0.039 mg/mL and 0.078 mg/mL, respectively. The inhibition of conidial germinationassay was performed at 0 × MIC, 0.5 × MIC, 1 × MIC, 2 × MIC, and 4 × MIC, and theresult showed G. candidum were completely inhibited at 4 × MIC. The ideal concentrationof P. cubeba L. extract on different selected food commodities were 0.50% against G.candidium in tomato, 0.50% to 5.00% against P. citrinum in grapes, and 5.00% against T.hirsuta in lemon. The findings implied that P. cubeba L. extract can be listed as one of thenatural antifungal agents in food. The availability of various types of natural antifungalagents will help in reducing the usage of chemical fungicide, which may lead to adverseand deteriorating health effects in the events of prolong consumption.