Open AccessBaccaurea racemosa (Reinw. ex Blume) Müll. Arg. pulp: a potential natural antioxidant
Author(s) -
Leidya Karina Permatasari,
Sugeng Riyanto,
Alumni Oke Octaviane Rohman
Publication year - 2019
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.3(6).165
Subject(s) - dpph , abts , chemistry , gallic acid , trolox , flavonoid , ethyl acetate , pulp (tooth) , food science , antioxidant , rutin , chromatography , botany , traditional medicine , organic chemistry , biology , medicine , pathology
Baccaurea racemosa (Reinw. ex Blume) Müll. Arg. is a fruit widely grown in Indonesialocally known as ‘kepundung’. Some of genus Baccaurea has potential to be developed asa natural antioxidant. However, the antioxidant activity of B. racemosa pulp has not beenpublished. This study is aimed to (i) explore the antioxidant activity, (ii) measure the totalphenolic and flavonoid contents, and (iii) investigate the correlation of antioxidant activityof extract and fractions of B. racemosa pulp with their total phenolic and flavonoidcontents. Fresh B. racemosa pulp was macerated with methanol to obtained methanolicextract. The methanolic extract was partitioned using n-hexane followed dichloromethaneand ethyl acetate. This study revealed that ethyl acetate fraction has the highest antioxidantactivity with the scavenging activity of free radical 2,2-diphenyl-1-picrylhydrazyl(DPPH) and 2,2’-Azinobis(3-etylbenzthiazoline)-6-sulfonic acid (ABTS) with valuesIC50 127.155±4.311ug/mL and 108.155±6.455 mM trolox equivalent/100 mg dry extractrespectively. However, the highest antioxidant activity using β-carotene bleaching (BCB)assay is the methanolic extract with value 36.898±13.240%. Furthermore, the ethyl acetatefraction has the highest total phenolic content (TPC) and total flavonoid content (TFC)with values 42.975±1.978 mg gallic acid equivalent/g dry extract (mg GAE g-1dry extract)and 122.813±1.604 mg rutin equivalent/g dry extract (mg RE g-1dry extract) respectively.TPC and TFC of B. racemosa pulp have a significant correlation with ABTS free radicalscavenging activity. In this current study, it was discovered that ethyl acetatefraction of B. racemosa pulp can be developed to be a natural antioxidant.