Open AccessAssessment of DNA damage through micronucleus studies in subjects exposed to formalin
Author(s) -
K Sriambika
Publication year - 2021
Publication title -
medpulse international journal anatomy/medpluse international journal of anatomy
Language(s) - English
Resource type - Journals
eISSN - 2636-4557
pISSN - 2550-7621
DOI - 10.26611/10011911
Subject(s) - micronucleus test , buccal swab , micronucleus , dna damage , comet assay , medicine , peripheral blood , pathology , carcinogen , toxicology , physiology , biology , microbiology and biotechnology , dna , toxicity , genetics
Background: Formaldehyde (FA) is the reactive and simplest of all the aldehydes. It is used as a preservative in anatomy, pathology and forensic laboratories. The international agency for research on cancer has classified FA as a carcinogen that can cause nasopharyngeal carcinoma, Leukaemia, Liver and pancreatic cancer. Objective And Method: The aim of the study was to assess the DNA damage in peripheral blood lymphocytes and in buccal cells by Micronucleus assay in Formalin exposed workers of Anatomy, Pathology and Forensic laboratories and compare with the control group, and also to analyze the relationship between frequency of Micronuclei and duration of exposure to formalin. Results: The mean and standard deviation (SD) of micronuclei in peripheral blood of exposed was 8.35 and in controls was 4.18. There was a significant increase in the frequency of MN in exposed group when compared with the comparison group (p<0. 5876). Pearson’s correlation test showed a positive correlation between the years of FA exposure and the number of micronuclei in buccal cells and peripheral blood indicating that DNA damage due to FA was directly proportional to the duration of exposure (r=0.8, 0.9). Conclusion: The present study was done to assess the DNA damage in people who were exposed to FA and a control group not exposed to FA by buccal cell and peripheral blood Micronucleus Assay. There was a significant increase in the MN in people exposed to FA which was directly proportional to the duration of exposure.