Open AccessOptimization of Antibacterial Production of Endophytic Fungi with Various Sources of C, N, and pH using The Response Surface Methodology
Author(s) -
Hary Widjajanti,
Elisa Nurnawati,
Muharni Muharni,
Eca Desriana Zahwa
Publication year - 2022
Publication title -
science and technology indonesia
Language(s) - English
Resource type - Journals
eISSN - 2580-4391
pISSN - 2580-4405
DOI - 10.26554/sti.2022.7.2.149-157
Subject(s) - secondary metabolite , plant use of endophytic fungi in defense , yeast extract , sucrose , response surface methodology , chemistry , antibacterial activity , food science , terpenoid , metabolite , composition (language) , phenols , yeast , nitrogen , botany , fermentation , chromatography , biology , biochemistry , bacteria , organic chemistry , genetics , linguistics , philosophy , gene
Secondary metabolites extract of McB1 endophytic fungi from gelam (Melaleuca cajuputi Powell.) leaves have a high potential antibacterial activity against Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538 with flavonoids and phenol as bioactive compound. The low production of secondary metabolites extract in the cultivation stage and the high potential antibacterial activity of bioactive compounds produced by McB1 endophytic fungi require special treatment for optimize the secondary metabolites product. This is possibly achieved by optimizing the composition of the cultivation media, where various sources of carbon, nitrogen, and pH produce different amounts and classes of secondary metabolites. The objectives of the research to obtain the optimum interaction between sources of carbon, nitrogen, and pH for the production of secondary metabolite extract using Response Surface Methodology (RSM). The results showed that the highest extract (0.25 g) with the composition of sucrose as carbon source, yeast extract as nitrogen source, and pH 6. Based on the optimization of the medium with a variation of 4.5 gL-1 sucrose, 0.48 gL-1 yeast extract, and pH 6.1 yielded 0.34 g of secondary metabolites extract of McB1 endophytic fungi. The chromatogram profile of the optimized secondary metabolite extract showed the presence of flavonoids, phenols, terpenoids, and tannins.