Open AccessCONSERVATION OF RARE MEDICINAL PLANT BY RAPID CALLUS INDUCTION FROM LEAF, NODE AND INTERMODAL EXPALNTS OF THALICTRUM JAVANICUM BLUME.
Author(s) -
Abinaya Gurunathan,
Paulsamy Subramanium
Publication year - 2017
Publication title -
kongunadu research journal
Language(s) - English
Resource type - Journals
ISSN - 2349-2694
DOI - 10.26524/krj212
Subject(s) - callus , explant culture , auxin , cytokinin , botany , biology , plant growth , shoot , murashige and skoog medium , horticulture , in vitro , biochemistry , gene
The callus culture of Thalictrum javanicum Blume was generated from leaf, node and internodal explants. Different growth regulators are greatly influenced in the growth of callus cultures. The callus was obtained from leaf, node and internodal explants by inoculating them on MS medium supplemented with various concentrations and combinations of growth regulators viz., auxins (IAA, NAA), and cytokinin (BAP) were used. The supplementation of medium with BAP (3.8) and IAA (0.1) was suitable for the efficient callus induction when compared with others.