Open AccessStress-Induced Accumulation of HnRNP K into Stress Granules
Author(s) -
Jayoung Kim,
Austin Yeon,
WoongKi Kim,
Khae Hawn Kim,
Takbum Ohn
Publication year - 2021
Publication title -
journal of cancer science and clinical therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2637-5079
DOI - 10.26502/jcsct.5079129
Subject(s) - mapk/erk pathway , stress granule , microbiology and biotechnology , gene silencing , clotrimazole , ribonucleoprotein , apoptosis , cytoplasm , chemistry , rna interference , biology , translation (biology) , kinase , rna , messenger rna , gene , genetics , antifungal
Stress granules (SGs) are cytoplasmic aggregates to reprogram gene expression in response to cellular stimulus. Here, we show that while SGs are being assembled in response to clotrimazole, an antifungal medication heterogeneous nuclear ribonucleoprotein (hnRNP) K, an RNA-binding protein that mediates translational silencing of mRNAs, is rapidly accumulated in SGs in U-2OS osteosarcoma cells. Forced expression of hnRNP K induces resistance to clotrimazole-induced apoptosis. Erk/MAPK is transiently activated in response to clotrimazole, and pharmacological suppression of the Erk/MAPK pathway sensitizes the cells to apoptosis. Inhibition of the Erk/MAPK pathway promotes the assembly of SGs. These results suggest that dynamic cytoplasmic formation of SGs and hnRNP K relocation to SGs may be defensive mechanisms against clotrimazole-induced apoptosis in U-2OS osteosarcoma cells.