Open AccessImproved expression of Catechol 1,2-dioxygenase gene from Burkholderia cepacia in Escherichia coli
Author(s) -
Van Thanh Dang,
Anh Thi Hoang,
Gia Cat Tuong Tran,
Thi Huyen Tran Pham,
Le Thi Ha Thanh,
Hoang Loc Nguyen
Publication year - 2020
Publication title -
tạp chí khoa học đại học huế: khoa học tự nhiên/tạp chí khoa học đại học huế: khoa học tự nhiên (online)
Language(s) - English
Resource type - Journals
eISSN - 2615-9678
pISSN - 1859-1388
DOI - 10.26459/hueunijns.v129i1d.5974
Subject(s) - escherichia coli , dioxygenase , burkholderia , dibenzofuran , inducer , chemistry , gene expression , enzyme , catechol , gene , lac operon , biochemistry , microbiology and biotechnology , biology , bacteria , genetics , organic chemistry
Catechol 1,2-dioxygenase (CAT1) is a key enzyme for the ortho-cleavage pathway involved in the degradation of dibenzofuran, a dioxin derivative, which is a highly toxic environmental pollutant. The present study aims to investigate appropriate culture conditions for enhancing the expression of the cat1 gene encoding CAT1 enzyme from Burkholderia cepacia DF4 in Escherichia coli M15. The optimized culture conditions for gene expression are cell density at the time of induction, shaking speed, induction temperature, induction time, and inducer concentration. The highest level for CAT1 was obtained at the IPTG concentration of 1.2 mM, 10 hours after induction at 35 °C, shaking speed 200 rpm with cell density at OD600 0.7.