Open AccessCLASSIFICATION OF SOME COMMERCIAL LINGZHI (Ganoderma spp.) ACCESSIONS IN VIETNAM BY USING ITS DNA BARCODE
Author(s) -
Hồ Viết Thế,
Võ Thị Ngọc Hà,
Lê Ngọc Giàu
Publication year - 2019
Publication title -
tạp chí khoa học đại học huế: khoa học tự nhiên/tạp chí khoa học đại học huế: khoa học tự nhiên (online)
Language(s) - English
Resource type - Journals
eISSN - 2615-9678
pISSN - 1859-1388
DOI - 10.26459/hueuni-jns.v128i1e.5380
Subject(s) - genetic diversity , barcode , biology , dna barcoding , ganoderma , fungus , genetic marker , genotype , identification (biology) , botany , genetic distance , range (aeronautics) , microbiology and biotechnology , genetic variation , food science , genetics , zoology , medicine , gene , ganoderma lucidum , environmental health , operating system , materials science , composite material , population , computer science
In Vietnam, lingzhi is mainly used for medicinal purposes with high market value leading to the increasing production in recent years. However, the identification and classification of this fungus is inaccurate due mainly to morphological characteristics. In this study, ITS-DNA barcode was used to analyze genetic composition of 10 commercial lingzhi genotypes collected from different regions across the country. The results showed that there is genetic diversity of 10 lingzhi samples. The genetic distance among studies accesson range from 0.000 to 0.047. Seven of ten accessions consisting of TG1, TG2, LD, DN, CC, DLk, and HN were reclassified as G. lingzhi and three accessions were identified as G. lucidum. In addition, 19 nucleotide sites differed among fungal samples were also reported. The results of this study show the high potential of ITS-DNA barcode for identification and classification as well as providing information on the genetic relationship between lingzhi varieties which could be applied as a basis for lingzhi breeding programs.