Open AccessVALIDATION AND QUANTITATIVE ANALYSIS OF LEAD AND CADMIUM IN DRIED STEVIA LEAVES BY GRAPHITE FURNACE ATOMIC ABSORPTION SPECTROPHOTOMETRY
Author(s) -
Dong Kim Nguyen,
Ngân Thi kim Lâm
Publication year - 2018
Publication title -
tạp chí khoa học đại học huế: khoa học tự nhiên/tạp chí khoa học đại học huế: khoa học tự nhiên (online)
Language(s) - English
Resource type - Journals
eISSN - 2615-9678
pISSN - 1859-1388
DOI - 10.26459/hueuni-jns.v127i1a.4406
Subject(s) - detection limit , cadmium , atomic absorption spectroscopy , chemistry , graphite furnace atomic absorption , repeatability , spectrophotometry , analytical chemistry (journal) , stevia , nuclear chemistry , chromatography , physics , medicine , alternative medicine , organic chemistry , quantum mechanics , pathology
A Graphite Furnace - Atomic Absorption Spectrophotometry (GF-AAS) for the quantification of cadmium (Cd) and lead (Pb) in dried stevia leaves were developed and validated. The method was validated for some parameters, namely linearity and range, precision, accuracy, Limit of Detection (LoD) and Limit of Quantification (LoQ), according to International Conference on Harmonization (ICH). The developed method was linear over the concentration ranging 0 - 2 µg/L (Cd) and 0 – 20 µg/L (Pb). The correlation coefficient (r) obtained for two metal are higher than 0.995. Limit of detection (LOD) of Cd and Pb was 0.12 µg/L, 0.33 µg/L. The recovery and repeatability in this method were good enough for determining these heavy metals in dried stevia leaves sample.The developed method has been applied successfully for determination of Cd and Pb levels in dried stevia leaves in Vietnam. Keywords: Stevia, Atomic Absorption Spectrophotometera (AAS), recovery, detection limit, validation.