Open AccessTHE ESTIMATION METHOD OF S-ACID LACTIC IN THE FERMENTATION PRODUCTS OF ALOE VERA GEL BY HIGH- PERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
Nguyen Kim Dong,
Duong Thi Bich,
Nguyen Chi Toan
Publication year - 2017
Publication title -
tạp chí khoa học đại học huế: khoa học tự nhiên/tạp chí khoa học đại học huế: khoa học tự nhiên (online)
Language(s) - English
Resource type - Journals
eISSN - 2615-9678
pISSN - 1859-1388
DOI - 10.26459/hueuni-jns.v126i1b.4260
Subject(s) - aloe vera , chromatography , lactic acid , chemistry , fermentation , high performance liquid chromatography , detection limit , correlation coefficient , biochemistry , bacteria , biology , microbiology and biotechnology , mathematics , genetics , statistics
An accurate, precise, simple and economical High Performance Liquid Chromatographic method for the estimation of S-acid lactic in the process of fermentation of Aloe Vera gel has been developed. The method is using HIQSIL 100 C18column (Length: 250nm, Diameter: 4.6nm, Particle size: 5μ) with potassium dihydrogen phosphate as mobile phase. The separation was performed by UV detector at 210 nm wavelength. The flow rate was kept at 1mL/min and the injection volume was 20μl. The results obtained showed that the linearity of the method was 0-180 ppm, and the correlation coefficient was found to be 0.999. Detection limit of the method for S-acid lactic was 1.15 ppm. The mean recoveries were from 95% to 99%. The reproducibility RSDs ranged from 2.87%. The results demonstrated the suitability of the HPLC approach for the analysis of S-acid lactic in the process of fermentation from Aloe Vera gel.