Open AccessDevelopment and validation for determination of lisinopril dihydrate in bulk drug and formulation using RP-HPLC method
Author(s) -
Zahid Zaheer,
Sarah Khan,
Mohammad Sadeque,
G. I. Hundekari,
Rana Zainuddin
Publication year - 2018
Publication title -
journal of inventions in biomedical and pharmaceutical sciences
Language(s) - English
Resource type - Journals
ISSN - 2456-818X
DOI - 10.26452/jibps.v3i2.1426
Subject(s) - lisinopril , chromatography , chemistry , phosphate buffered saline , acetonitrile , high performance liquid chromatography , pharmaceutical formulation , dosage form , angiotensin converting enzyme , medicine , blood pressure , radiology
A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for Lisinopril in bulk drug and formulation. A column having 150 × 4.6 mm in isocratic mode with mobile phase containing acetonitrile: phosphate buffer (70:30; adjusted to pH 3.0) was used. The flow rate was 0.8 ml/min and effluent was monitored at 216 nm. The retention time (min) and linearity range (μg/ml) for Lisinopril was (1.510) and (10-35). The developed method was found to be accurate, precise and selective for determination of Lisinopril in bulk and formulation.