Open AccessInfluence of biosynthesized silver nanoparticles on keratinase activity and mycelialgrowth of dermatophytes
Author(s) -
Adnan Hamad Uobeed Al Hamadani,
Balsam Miri Mizher Al Muhna
Publication year - 2018
Publication title -
international journal of research in pharmaceutical sciences
Language(s) - English
Resource type - Journals
ISSN - 0975-7538
DOI - 10.26452/ijrps.v9ispl1.1361
Subject(s) - aspergillus niger , broth microdilution , mycelium , silver nanoparticle , chemistry , fourier transform infrared spectroscopy , microbiology and biotechnology , minimum inhibitory concentration , nuclear chemistry , antimicrobial , biology , nanoparticle , food science , materials science , nanotechnology , botany , chemical engineering , engineering
Among different nanomaterial,the silver nanoparticles (SNPs) exhibited high antifungal potency compared with other types of nanoparticles (NPs),and this property is often very helpful,particularly against fungi resistant to conventional antifungal agents. However synthesis of SNPs can generate toxic waste during the preparation process. Accordingly,new technique using non-toxic routs have been researched for synthesis of SNPs using cell-free filtrate of Aspergillus niger and evaluate their effect against some dermatophytes spp.The proposal of our study was to biosynthesize SNPs using cell-free filtrate of Aspergillus niger as reducing agent. The characterization of biosynthesized SNPs were carried out by UV-Visible spectroscopy,Fourier transform infrared spectroscopic analysis (FTIR) and scanning electron microscopy (SEM).The antifungal effect of the NPs against dermatophytes was also evaluated. The minimum inhibitory concentration (MIC) was determined by broth microdilution method. pherical NPs 15-50 nm in size were obtained.The biosynthesized SNPs exerted pronounced morphological alteration in the fungal mycelia.Additionally,the inhibition of keratinase activity of the tested dermatophytes was also recorded.The results indicate that SNps can be synthesized in ecofriendly,inexpensive and promising technique by fungal strain of A.niger,and it has considerable antifungal activity comparison with other antifungal drugs.