Open AccessInduction of apoptosis by selected Xylocarpus sp.,fractions in the human cervical cancer cell line, HeLa
Author(s) -
Gul-e-Saba Chaudhry,
Nur Khairina Ahmed Sohimi,
Habsah Mohamad,
Muhammad Zafar,
Aziz Ahmed,
Yeong Yik Sung,
Tengku Sifzizul Tengku Muhammad
Publication year - 2020
Publication title -
international journal of research in pharmaceutical sciences
Language(s) - English
Resource type - Journals
ISSN - 0975-7538
DOI - 10.26452/ijrps.v11i2.2210
Subject(s) - dna fragmentation , apoptosis , hela , traditional medicine , chemistry , programmed cell death , tunel assay , fragmentation (computing) , pharmacology , biology , biochemistry , cell , medicine , ecology
Cancer is unchecked growth of normal cells. The screening of new potential agents is vital to develop effective cytotoxic drugs with reduced side effects. Thecytotoxic effects, mode of cell death and screening of phytochemicals which induced cell death by the mangrove plant Xylocarpus granatum extract on the human hepatocellular carcinoma cell line was studied. The crude methanol extract of Xylocarpusgranatum(bark) partitioning produced the high yield in diethyl ether (54.08%)followed by butanol (37.12%) and aqueous (3.21%).The Xylocarpus granatum bark extract produced a cytotoxic effect with IC50 100 µg/ml). The DNA fragmentation study done to determine the mode of cell death. The results attained by the colorimetric TUNEL system propose that Xylocarpus moluccensis extract induced DNA fragmentation in the HepG2 cell line. The darkly stained nuclei observed in HepG2cells treated with Xylocarpus moluccensis(diethyl ether) extract for 24 hours. The DNA fragmentation indicating that apoptosis was the mode of cell death on HepG2 cell line. Furthermore, The Xylocarpus moluccensis(diethyl ether) extracts phytochemical study confirms the presence of flavonoid, steroid, terpenoid, phenols, tannins, glycoside, and saponin.Thus, thefurther study of potential phytochemicalsneed to done in order to confirm these phytochemicalsas a future therapeutic agent for the treatment of cancer.