Open AccessGenotoxic property of poly herbal formulation of annona squomosa, zingiber officinalis and triticum aestivum plant extracts by in vitro chromosomal aberration test
Author(s) -
Rajaian Pushpabai Rajesh,
Jagadeesh Singh S D,
R Channabasava
Publication year - 2019
Publication title -
international journal of research in pharmaceutical sciences
Language(s) - English
Resource type - Journals
ISSN - 0975-7538
DOI - 10.26452/ijrps.v10i4.1662
Subject(s) - chromosome aberration , officinalis , traditional medicine , biology , genotoxicity , botany , chemistry , medicine , chromosome , biochemistry , toxicity , gene , organic chemistry
The present study was aimed to evaluate the genotoxic potential of polyherbal formulations by chromosomal aberration test. Cancer being the second most cause of death among all ailments even after the improvised medications needs alternative sources of treatment. Herbal sources were always among the one as prime treatment sources. Polyherbal formulations were taking the lead in medications because of their broad spectrum of activity and synergic effects. Annona squamosa, Zingiber Officinalis, and Triticum Aestivum formulation with 1:2:3 ratio has shown significant results in the definitive and confirmatory chromosome aberration assays. Indicated the test article PF3 did not induce a statistically significant increase in the percentage of cells with aberrations both in the presence and absence of metabolic activation. PF3 was found non-clostagenic at a maximum dose of 15 µg/ml in the CHO cell line. Inhibition of chromosomal aberration, DNA fragmentation, and maintenance of cellular integrity may prevent the genotoxic effect. Further optimization and in vivo authenticated studies need to be proven.