Open AccessIsolation and characterization of components from roots of Premna latifolia Roxb
Author(s) -
M. Gowtham,
Indira Viswambaran Asharani,
M Paridhavi
Publication year - 2019
Publication title -
international journal of research in pharmaceutical sciences
Language(s) - English
Resource type - Journals
ISSN - 0975-7538
DOI - 10.26452/ijrps.v10i3.1463
Subject(s) - column chromatography , chemistry , proton nmr , carbon 13 nmr , alcohol , thin layer chromatography , stereochemistry , chromatography , organic chemistry
The aim of the current work was to isolate and characterize structurally fascinating and biologically intriguing compounds from the dried roots of Premna latifolia. Dried plant roots were subjected to soxhalation with ethyl alcohol and later to column chromatography. The individual compounds were isolated by preparative thin-layer chromatography followed by structural characterization using various spectral ways like LCMS, IR, 1D-NMR and 2D-NMR (1H-1H NMR and 1H-13C NMR). Three totally different compounds were isolated and characterised as Compound 1: n-Tridecanyl n-Tetracosanoate, Compound 2: Stigmanstan -3β-olyln-octadec-9ʹ, 12ʹ-dienoate and Compound 3: n-Tetracosanol for the first time from Premna latifolia roots.