Open AccessUJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JERUK SAMBAL (Citrus x hystryx) TERHADAP Escherichia coli DAN Staphylococcus aureus
Author(s) -
Yurisa Yurisa,
Muhammad Agus Wibowo
Publication year - 2019
Publication title -
indonesian journal of pure and applied chemistry
Language(s) - English
Resource type - Journals
eISSN - 2620-858X
pISSN - 2620-8571
DOI - 10.26418/indonesian.v2i2.36905
Subject(s) - essential oil , staphylococcus aureus , antibacterial activity , linalool , chemistry , escherichia coli , traditional medicine , food science , bacteria , biology , medicine , biochemistry , genetics , gene
Essential oils of Jeruk Sambal leaves (Citrus x hystryx) have as antibacterial agents. This study aims to determine the antibacterial activity of dried Jeruk Sambal leaves originating from Sungai Kakap area against Escherichia coli and Staphylococcus aureus. Stages in this study were divided into two stages (1) isolation of volatile oil by steam distillation, (2) test the antibacterial activity by pitting diffusion method. Essential oil yield of 0.22% was obtained. The results of GC-MS analysis showed that the essential oil of Jeruk Sambal leaves dried has 4 main compounds, namely elemol (26.27%), β-eudesmol (14.47%), 2-β-pinene (13.84%) and linalool (7.77%). The test results showed that the antibacterial activity of essential oils of Jeruk Sambal leaves was more active in inhibiting the growth of S. aureus than E. coli bacteria.