Open AccessFlow-based Methods in Chemical Peptide and Protein Synthesis
Author(s) -
Kevin Schiefelbein,
Nina Hartrampf
Publication year - 2021
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2021.480
Subject(s) - peptide , flow chemistry , chemistry , computer science , peptide synthesis , flow (mathematics) , combinatorial chemistry , biochemical engineering , nanotechnology , chemical synthesis , continuous flow , data science , computational biology , biochemistry , engineering , biology , materials science , geometry , mathematics , in vitro
Flow chemistry has emerged as a powerful method for on-demand chemical synthesis and modification of peptides and proteins. Herein, we discuss the characteristics of flow chemistry and how they are applied to various aspects of peptide chemistry. We highlight recent advances in automated flow-based peptide synthesis, which extend the length of peptides routinely accessible to single-domain proteins and allow for the collection of time-resolved synthesis data. Applications of this data for the prediction of synthesis outcome and the potential for the development of more sustainable synthesis methods are also discussed. Finally, we will review solutionphase approaches, including flow-based ligation strategies and peptide cyclization. Throughout this review, the current challenges and potential future developments are highlighted.