Open AccessAquaSparkTM – Rapid Environmental Monitoring of Listeria monocytogenes
Author(s) -
Giverny Ganz,
Lukas Reinau,
Anne-Flore Imhaus,
Mario Hupfeld,
Lars Fieseler
Publication year - 2020
Publication title -
chimia
Language(s) - English
Resource type - Journals
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2020.791
Subject(s) - listeria monocytogenes , contamination , food safety , listeria , biochemical engineering , microbiology and biotechnology , human health , risk analysis (engineering) , bacteria , biology , food science , business , medicine , engineering , environmental health , ecology , genetics
In order to prevent microbial contamination of food, monitoring of the production environment, together with the rapid detection of foodborne pathogens have proven to be of utmost importance for Food Safety. Environmental monitoring should detect harmful pathogens at the earliest point in time in order for the necessary interventions to be taken. However, current detection methods fall short with regards to speed, ease of use, and cost. This article aims to present the idea behind NEMIS Technologies, a startup company making use of the novel AquaSparkTM technology for the development of a new generation of bacterial detection methods. These methods utilize chemiluminescence in order to detect live target bacteria in a short period of time compared to that of conventional methods. We show that dry-stressed Listeria monocytogenes can be detected within 24 hours, using small-molecule chemiluminescent probes, together with a bacteria-specific proprietary enrichment broth containing a cocktail of bacteriophages, which enhance the specificity and sensitivity. This novel platform technology has the potential to extend beyond environmental monitoring towards food analyses as well as veterinary and human health.