Open AccessDesign of in vitro Transcribed mRNA Vectors for Research and Therapy
Author(s) -
Marina Tusup,
Lars E. French,
Mara De Matos,
David Gatfield,
Thomas M. Kündig,
Steve Pascolo
Publication year - 2019
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2019.391
Subject(s) - messenger rna , in vitro , genetic enhancement , untranslated region , rna , transfection , translation (biology) , microbiology and biotechnology , biology , open reading frame , computational biology , gene , genetics , peptide sequence
The use of in vitro transcribed messenger RNA (ivt mRNA) for vaccination, gene therapy and cell reprograming has become increasingly popular in research and medicine. This method can be used in vitro (transfected in cells) or administered naked or formulated (lipoplexes, polyplexes, and lipopolyplexes that deliver the RNA to specific organs, such as immune structures, the lung or liver) and is designed to be an immunostimulatory or immunosilent agent. This vector contains several functional regions (Cap, 5' untranslated region, open reading frame, 3' untranslated region and poly-A tail) that can all be optimised to generate a highly efficacious ivt mRNA. In this study, we review these aspects and report on the effect of the ivt mRNA purification method on the functionality of this synthetic transient genetic vector.