Open AccessModern Ligation Methods to Access Natural and Modified Proteins
Author(s) -
Alice Baumann,
Christian P. R. Hackenberger
Publication year - 2018
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2018.802
Subject(s) - ligation , chemical ligation , peptide bond , peptide , native chemical ligation , drug discovery , chemistry , computational biology , combinatorial chemistry , computer science , nanotechnology , biochemistry , chemical synthesis , biology , microbiology and biotechnology , materials science , in vitro
Proteins and peptides are gaining increasing interest as tools and targets in fundamental research and drug discovery. Growing research applications have prompted the need for methodologies that produce homogenous peptide and protein material. The development of efficient, chemoselective ligation reactions using unprotected peptide fragments presents a key solution for this challenging task. This review outlines modern ligation methods that enable the synthesis of both native, and also labelled or post-translationally modified peptides and proteins. The ligation methods herein discussed focus on the formation of the backbone amide bond.