Open AccessInduction of Intracellular Reductive Stress with a Photoactivatable Phosphine Probe
Author(s) -
Alina Tirla,
Pablo RiveraFuentes
Publication year - 2018
Publication title -
chimia
Language(s) - English
Resource type - Journals
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2018.241
Subject(s) - intracellular , chemistry , phosphine , phosphonium , biophysics , fluorescence , reductive elimination , membrane , combinatorial chemistry , biochemistry , biology , organic chemistry , catalysis , physics , quantum mechanics
Reductive stress is a condition present in cells that have an increased concentration of reducing species, and it has been associated with a number of pathologies, such as neurodegenerative diseases and cancer. The tools available to study reductive stress lack both in selectivity and specific targeting and some of these shortcomings can be addressed by using photoactivatable compounds. We developed a photoactivatable phosphonium probe, which upon irradiation releases a fluorescent molecule and a trialkyphosphine. The probes can permeate through the plasma membrane and the photoreleased phosphine can induce intracellular reductive stress as proven by the detection of protein aggregates.