Open AccessTwo- and Three-dimensional Mid-Infrared Chemical Imaging
Author(s) -
Fabio Zobi,
Martin Obst
Publication year - 2017
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2017.32
Subject(s) - chemical imaging , focus (optics) , fourier transform infrared spectroscopy , infrared , microscopy , tomography , tomographic reconstruction , nanotechnology , optics , materials science , computer science , physics , artificial intelligence , hyperspectral imaging
This article describes the state-of-the-art in 2D and 3D tomographic mid-IR imaging and its current and potential applications in the imaging of material and biological systems with a focus on cells and tissues. 2D FTIR microscopy is first presented in terms of the basic experimental configurations of the technique, optics, data and image acquisition. This first section provides a basis for the ensuing discussion on 3D FTIR spectro-microtomography. In the second part, examples of 2D and 3D chemical imaging are given with a focus on the use of molecular chemical probes and probe-free applications, respectively.