Open AccessComparison of UHPLC-ESI-MS and Hadamard Transform Atmospheric Pressure Ion Mobility-ESI-MS for Rapid Profiling of Isomeric Flavonoids
Author(s) -
Michael Groessl,
Antonio Azzollini,
Philippe J. Eugster,
Benoît Plet,
JeanLuc Wolfender,
Richard Knochenmuss
Publication year - 2014
Publication title -
chimia
Language(s) - English
Resource type - Journals
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2014.135
Subject(s) - chemistry , chromatography , ion mobility spectrometry , hadamard transform , glycoside , atmospheric pressure , flavonoid , analytical chemistry (journal) , mass spectrometry , stereochemistry , organic chemistry , antioxidant , mathematical analysis , oceanography , mathematics , geology
Hadamard transform atmospheric pressure ion mobility-MS and rapid UHPLC-MS methods were investigated for analysis of closely related isomeric flavonoids and their glycosides using a test set of seven standards. On a time scale of a few minutes, the flavonoid aglycones were all separated by ion mobility, but not by UHPLC. The glycosides were better resolved by IMS but not completely separated by both methods. The results suggest that IMS provides sufficient resolution for separation of isomeric polyphenols such as flavonoids in high-throughput metabolomics studies.