Open AccessVitamin E and Vitamin E Acetate Absorption from Self-assembly Systems under Pancreas Insufficiency Conditions
Author(s) -
Kornél Nagy,
Beatriz Lobo,
Marie-Claude Courtet-Compondu,
Sophie Braga-Lagache,
Laura Ramos,
Valentí Puig-Diví,
Fernando Azpiroz,
Juan R. Malagelada,
Maurice Beaumont,
Julie Moulin,
Simone Acquistapace,
Laurent Sagalowicz,
Martin Kussmann,
Javier Santos,
Birgit Holst,
Gary Williamson
Publication year - 2014
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2014.129
Subject(s) - bioavailability , vitamin e , chemistry , cmax , absorption (acoustics) , micelle , tocopherol , chromatography , vitamin , antioxidant , organic chemistry , materials science , biochemistry , pharmacology , aqueous solution , biology , composite material
We determined the bioavailability of vitamin E from self-assembly structures in patients with diagnosed chronic pancreas insufficiency. Vitamin E solubilized in dispersed inverted bicontinuous cubic phase and in micellar formulation was delivered directly to the small intestine by tube-feeding. A cross-over study with randomization of 6 subjects and 2 treatments including a combined dose of 18 mg (27 IU) of vitamin E (RRR-[5,7-methyl-(2H6)]-?-tocopherol) and 27 mg (27 IU) vitamin E acetate (RRR-[5-methyl-2H3]-?-tocopheryl acetate) was applied over a time period of 1 h. Plasma samples were collected for 56 h and analyzed by liquid chromatography–mass spectrometry. Appearance of labeled tocopherols originating from the treatment started at 25 h and reached Cmax (0.6–4.6 ?M depending on subject) in the 7–9 h window. From the Tmax onwards, both forms of tocopherols diminished slowly to 30–50% of their maxima within 56 h. Strong inter-individual variation was observed in the plasma appearance curves (relative standard deviation varied between 38–45%). No significant discrimination was found between the absorption of free or acetylated forms of deuterated ?-tocopherol confirming that application of acetylated ?-tocopherol provides the same bioavailability as free ?-tocopherol. This observation is valid in both dispersed inverted bicontinuous cubic phase and micellar formulations. Furthermore, since the area-under-the-curve values from cubic phase and from micellar formulations are similar, the cubic phase formulation could represent an alternative delivery system for lipophilic micronutrients in conditions or studies where polysorbate-based micelles cannot be generated.