Construction of a Peptide Microarray for Auto-anti- body Detection | Zendy

Vincent Cosandey | Zendy; Fabien Debrot | Zendy; Jérémy Kaeser | Zendy; Roger Martí | Zendy; Philippe Passeraub | Zendy; Jannick Pétremand | Zendy; Denis Prim | Zendy; M Pfeifer | Zendy

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Construction of a Peptide Microarray for Auto-anti- body Detection

Author(s) -

Vincent Cosandey,

Fabien Debrot,

Jérémy Kaeser,

Roger Martí,

Philippe Passeraub,

Jannick Pétremand,

Denis Prim,

M Pfeifer

Publication year - 2012

Publication title -

chimia international journal for chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.387

H-Index - 55

eISSN - 2673-2424

pISSN - 0009-4293

DOI - 10.2533/chimia.2012.803

Subject(s) - multiplex , antibody microarray , protein microarray , peptide , microtiter plate , microarray , protein array analysis , microbiology and biotechnology , antibody , serology , dna microarray , antigen , computational biology , chemistry , biology , biochemistry , bioinformatics , gene , immunology , gene expression

Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).

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