Open AccessConstruction of a Peptide Microarray for Auto-anti- body Detection
Author(s) -
Vincent Cosandey,
Fabien Debrot,
Jérémy Kaeser,
Roger Martí,
Philippe Passeraub,
Jannick Pétremand,
Denis Prim,
M. Pfeifer
Publication year - 2012
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2012.803
Subject(s) - multiplex , antibody microarray , protein microarray , peptide , microtiter plate , microarray , protein array analysis , microbiology and biotechnology , antibody , serology , dna microarray , antigen , computational biology , chemistry , biology , biochemistry , bioinformatics , gene , immunology , gene expression
Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).