Open AccessVisualizing Biochemical Activities in Living Cells through Chemistry
Author(s) -
Luc Reymond,
Gražvydas Lukinavičius,
Keitaro Umezawa,
Damien Maurel,
Matthias A. Brun,
Anastasiya Masharina,
Karolina Bojkowska,
Birgit Mollwitz,
Alberto Schena,
Rudolf Griss,
Kai Johnsson
Publication year - 2011
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2011.868
Subject(s) - living cell , chemistry , computational biology , living systems , nanotechnology , microbiology and biotechnology , biology , computer science , materials science , artificial intelligence
The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over the last years, our laboratory has developed different labeling approaches for monitoring protein activity and for localizing synthetic probes inside living cells. In this article, we review two of these labeling approaches, the SNAP-tag and CLIP-tag technologies, and their use for studying cellular processes.