Open AccessGlycosidases in Carbohydrate Synthesis: When Organic Chemistry Falls Short
Author(s) -
Pavla Bojarová,
Vladimı́r Křen
Publication year - 2011
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2011.65
Subject(s) - glycoside hydrolase , chemistry , hydrolysis , stereoselectivity , catalysis , carbohydrate , oligosaccharide , enzyme , biocatalysis , organic synthesis , substrate specificity , combinatorial chemistry , biochemistry , reaction mechanism
Thanks to the stability, good availability, stereoselectivity and broad substrate specificity, oligosaccharide synthesis catalyzed by glycosidases represents an elegant way to complex carbohydrate structures. Two approaches to glycosidase catalysis are presented: i) the use of structurally modified substrates that carry various functional moieties in the molecule, and ii) the design of mutant glycosidases void of hydrolytic activity. Products of glycosidase-catalyzed synthesis are applicable in a range of are as such as immunology, therapy of Alzheimer's or Parkinson's diseases and the synthesis of neoglycoproteins.