Open AccessFluorescent Probes and Labels for Cellular Imaging
Author(s) -
Christoph J. Fahrni
Publication year - 2009
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2009.714
Subject(s) - fluorescence , nanotechnology , reagent , zinc , trace (psycholinguistics) , metal ions in aqueous solution , chemistry , metal , materials science , optics , linguistics , physics , philosophy , organic chemistry
Metal-responsive fluorescent indicators are powerful tools for visualizing trace metals with subcellular resolution. By taking advantage of the diverse photophysical properties of organic fluorophores, metal ion-selective fluorescent indicators have been rationally designed and tailored towards cellular applications. This review summarizes challenges associated with the probe design and describes recent efforts in our research group in developing selective and sensitive reagents for the detection of zinc and copper in mammalian cells.